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Axio Imager - 3D imaging excellence |
The main focus in modern fluorescence microscopy is the selective and specific imaging of information from all dimensions of a sample. The challenge here is to eliminate stray light from regions outside the selected focus planes. We offer users three systems, each perfectly tailored to your specific application. Together with precise z-motorisation and rapid, sample-protecting image acquisition, these systems are essential for achieving glare-free optical sections, optimum contrast and excellent visualisation in 3D.
3D mathematically – AxioVision 3D Deconvolution
 The powerful AxioVision 3D Deconvolution software mathematically calculates the stray light from outside the focus plane back to its point of origin. In this way, the object acquired in the 3D image stack is arithmetically “unfolded”. The result is first-class image quality especially in samples with very weak fluorescence.
3D award winner – ApoTome
The ApoTome is the solution if you are looking for sensational image quality with an instant display for tissue and thicker samples.  It is simply installed in the plane of the field diaphragm of the reflected light beam path of the Axio Imager D1 and Z1. ApoTome uses the principle of fringe projection to create precise optical sections online with increased contrast and greatly improved axial resolution. The ApoTome was recognised in 2003 with the R&D 100 and the Photonics Circle of Excellence Awards.
3D High-end – the LSM family
Confocal laser scanning microscopy is high-end technology in fluorescence. Regardless of which LSM application is used, Axio Imager provides the right platform for a powerful LSM workstation, whether with solo scanning heads or combined ones for effective synergy.
- The new LSM LIVE systems enable revolutionary
 scanning speeds in excess of 100 fps at full resolution and even higher sensitivity for current applications in live cell imaging.
- Uniquely, the LSM META system allows you to record even spectrally resolved image information, precisely separating fluorescence signals even when the emission spectra of the stains almost completely overlap.
- The LSM ConfoCor systems make it possible to quantitatively image individual molecular dynamics.
- The LSM NLO systems offer worldwide leading multiphoton technology.
The motorised focus drive – precision in the z-direction
 When it comes to z-motors, Axio Imager offers you two different choices. The standard solution has a step size of 25 nm and a reproducibility of ± 25 nm. For testing applications, such as confocal microscopy or for the creation of z-stacks with extremely small increments, a step size of 10 nm with a reproducibility of ± 10 nm is offered – all this and at three times the speed!
The closed loop system – highest focus precision
If you need to meet extremely high demands, the Axio Imager Z1 with the focus linear sensor offers you accuracy of ± 1 nm in the z-direction. The sensor detects the application-independent movements of the microscope stage and makes corresponding adjustments while performing extremely precise and reproducible z-stacks with equidistant step sizes. It's what we call maximum control and certainty.
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