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The use of SGE's exceptionally inert and thermally stable BPX5, BPX35 and BPX50 capillary columns (respectively 5%, 35% and 50% phenyl equivalent) for the analysis of organophosphorous pesticides has solved many of the problems associated with the chromatography of this class of compounds.
Organophosphorous pesticide analysis can be difficult and time consuming. High temperatures are usually required for the chromatography of many pesticides, causing problems with the bleed profile of the column and contaminants being left behind. Sample throughput and hence laboratory efficiency are further reduced by lengthy analysis times - often up to 40 minutes.
SGE's BPX5, BPX35 and BPX50 columns can analyse the US EPA 8141 mix (Table 1) in less than 22 minutes, allowing a higher throughput of samples. The baseline resolution of the organophosphorous pesticides is excellent on BPX5, BPX35 and BPX50 with the bleed profile a strong feature at temperatures up to 360°C.
The high thermal stability of the phase for each of these columns allows the analyst to "bake out" any high boiling contaminants to 360°C without damaging the stationary phase. This insures against the possibility of any interference from these contaminants with further analyses.
BPX5, BPX35 and BPX50 columns show outstanding inertness with even the most difficult of organophosphorous compounds; Naled, which readily breaks down to Dichlorvos on column, is easily detected without any apparent decomposition. This analysis has been performed on columns with dimensions of 30m x 0.25mm ID x 0.25µm which is a standard dimension column in most laboratories.
| BPX5 replaces |
BPX35 replaces |
BPX50 replaces |
| DB-5 |
DB-35 |
OV-17 |
| DB-5MS |
DB-35MS |
SP-2250 |
| DB-5.625 |
Rtx-35 |
DB-17 |
| XTI-5 |
Rtx-35ms |
DB-17MS |
| Rtx-5ms |
HP-35 |
DB-17ht |
| Ultra-2 |
HP-35MS |
Rtx-50 |
| HP-5 |
SPB-35 |
SPB-50 |
| HP-5MS |
MDN-35 |
HP-50+ |
| HP5-TA |
AT-35 |
HP-17 |
| SPB-5 |
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AT-50 |
| MDN-5S |
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007-17 |
| CP-Sil 8CB |
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| Trx-5Sil MS |
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| AT-5 |
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| CP-Sil 8CB M |
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BPX5, BPX35 and BPX50 columns show unparalleled performance for the separation of the phosphorylated pesticides listed in the US EPA 8141 method. They can be 'baked out' at the end of each analysis to remove any high boiling point contaminants without any degradation to the stationary phase and give excellent response and very little column breakdown. BPX5, BPX35 and BPX50 are the columns of choice for all organophosphorous pesticide analyses.
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